Description
Aviva Systems Biology Dio1 ELISA Kit (Mouse) (OKEH06590) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dio1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dio1 and incubated. The Dio1 found in the sample or standards competes with the biotinylated Dio1 for limited binding sites on the immobilized anti-Dio1 antibody. Excess unbound biotinylated Dio1 and sample or standard Dio1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dio1 captured in the well and inversely proportional to the amount of Dio1 which was contained in the sample or standard